Hiller M A, Lin T Y, Wood C, Fuller M T
Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305-5329, USA.
Genes Dev. 2001 Apr 15;15(8):1021-30. doi: 10.1101/gad.869101.
Alternate forms of the general transcription machinery have been described in several tissues or cell types. However, the role of tissue-specific TBP-associated factors (TAF(II)s) and other tissue-specific transcription components in regulating differential gene expression during development was not clear. Here we show that the cannonball gene of Drosophila encodes a cell type-specific homolog of a more ubiquitously expressed component of the general transcription factor TFIID. cannonball is required in vivo for high level transcription of a set of stage- and tissue-specific target genes during male gametogenesis. Regulation of transcription by cannonball is absolutely required for spermatogenesis, as null mutations block meiotic cell cycle progression and result in a complete failure of spermatid differentiation. Our results demonstrate that cell type-specific TAF(II)s play an important role in developmental regulation of gene expression.
在多种组织或细胞类型中已描述了通用转录机制的不同形式。然而，组织特异性TBP相关因子（TAF(II)s）和其他组织特异性转录成分在发育过程中调节差异基因表达的作用尚不清楚。在此我们表明，果蝇的炮弹基因编码通用转录因子TFIID中一种更广泛表达成分的细胞类型特异性同源物。在雄性配子发生过程中，炮弹基因在体内对于一组阶段和组织特异性靶基因的高水平转录是必需的。精子发生绝对需要炮弹基因对转录进行调控，因为无效突变会阻断减数分裂细胞周期进程，并导致精子细胞分化完全失败。我们的结果表明，细胞类型特异性TAF(II)s在基因表达的发育调控中起重要作用。
