Kaltenbach L, Horner M A, Rothman J H, Mango S E
Huntsman Cancer Institute Center for Children and Department of Oncological Sciences, University of Utah, Salt Lake City 84112, USA.
Mol Cell. 2000 Sep;6(3):705-13. doi: 10.1016/s1097-2765(00)00068-x.
Metazoans possess two TATA-binding protein homologs, the general transcription factor TBP and a related factor called TLF. Four models have been proposed for the role of TLF in RNA polymerase II (Pol II) transcription: (1) TLF and TBP function redundantly, (2) TLF antagonizes TBP, (3) TLF is a tissue-specific TBP, or (4) TLF and TBP have distinct activities. Here we report that CeTLF is required to express a subset of Pol II genes and associates with at least one of these genes in vivo. CeTLF is also necessary to establish bulk transcription during early embryogenesis. Since CeTLF and CeTBP are expressed at comparable levels in the same cells, these findings suggest CeTLF performs a unique function in activating Pol II transcription distinct from that of CeTBP.
后生动物拥有两种TATA结合蛋白同源物，即通用转录因子TBP和一种名为TLF的相关因子。关于TLF在RNA聚合酶II（Pol II）转录中的作用，已提出了四种模型：（1）TLF和TBP功能冗余，（2）TLF拮抗TBP，（3）TLF是组织特异性TBP，或（4）TLF和TBP具有不同的活性。在此我们报告，CeTLF是表达Pol II基因的一个子集所必需的，并且在体内与这些基因中的至少一个相关联。CeTLF对于早期胚胎发育过程中整体转录的建立也是必需的。由于CeTLF和CeTBP在相同细胞中以相当的水平表达，这些发现表明CeTLF在激活Pol II转录中执行与CeTBP不同的独特功能。
