Whitney A C, Clark A S
Department of Psychological and Brain Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA.
Biol Reprod. 2001 May;64(5):1460-5. doi: 10.1095/biolreprod64.5.1460.
The effects of anabolic-androgenic steroid (AAS) abuse on the onset of puberty in female adolescents are largely unknown. This study assessed the acute effects of one AAS, stanozolol, on pubertal onset in the female rat. A single injection of stanozolol (5 mg/kg) on Postnatal Day (PN) 21 advanced vaginal opening but did not alter the onset of vaginal estrus. Higher doses of stanozolol treatment (10 and 25 mg/kg) also advanced vaginal opening but had no effect on vaginal estrus. The advancement of vaginal opening by stanozolol (5 mg/kg) was prevented by the concomitant administration of the pure antiestrogen ICI 182,780 (1 mg/kg) on PN20-22. Administration of the androgen receptor antagonist flutamide (10 mg/kg twice daily) on PN20-22 had no effect on the advancement of vaginal opening by stanozolol. Stanozolol treatment also advanced vaginal opening in ovariectomized rats. Perivaginal injections of a low dose of stanozolol (0.05 mg) on PN21 and PN23 also advanced vaginal opening. These results suggest that stanozolol is acting directly at estrogen receptors in the vaginal epithelium to advance vaginal opening and that prepubertal stanozolol treatment does not induce true precocious puberty.
合成代谢雄激素类固醇（AAS）滥用对女性青少年青春期启动的影响在很大程度上尚不清楚。本研究评估了一种AAS司坦唑醇对雌性大鼠青春期启动的急性影响。在出生后第21天（PN21）单次注射司坦唑醇（5毫克/千克）可使阴道开口提前，但并未改变阴道发情的开始时间。更高剂量的司坦唑醇治疗（10和25毫克/千克）也使阴道开口提前，但对阴道发情没有影响。在PN20 - 22同时给予纯抗雌激素ICI 182,780（1毫克/千克）可阻止司坦唑醇（5毫克/千克）导致的阴道开口提前。在PN20 - 22给予雄激素受体拮抗剂氟他胺（10毫克/千克，每日两次）对司坦唑醇导致的阴道开口提前没有影响。司坦唑醇治疗也使去卵巢大鼠的阴道开口提前。在PN21和PN23经阴道周围注射低剂量司坦唑醇（0.05毫克）也使阴道开口提前。这些结果表明，司坦唑醇直接作用于阴道上皮中的雌激素受体以提前阴道开口，并且青春期前的司坦唑醇治疗不会诱导真正的性早熟。
