Bezabeh T, Mowat M R, Jarolim L, Greenberg A H, Smith I C
Institute for Biodiagnostics, National Research Council, Winnipeg, Manitoba, R3B 1Y6 Canada.
Cell Death Differ. 2001 Mar;8(3):219-24. doi: 10.1038/sj.cdd.4400802.
Apoptosis and necrosis need to be differentiated in order to distinguish drug-induced cell death from spontaneous cell death due to hypoxia. The ability to differentiate between these two modes of cell death, especially at an early stage in the process, could have a significant impact on accessing the outcome of anticancer drug therapy in the clinic. Nuclear magnetic resonance spectroscopy was used to distinguish apoptosis from necrosis in human cervical carcinoma (HeLa) cells. Apoptosis was induced by treatment with the topoisomerase II inhibitor etoposide, whereas necrosis was induced by the use of ethacrynic acid or cytochalasin B. We found that the intensity of the methylene resonance increases significantly as early as 6 h after the onset of apoptosis, but that no such changes occur during necrosis. The spectral intensity ratio of the methylene to methyl resonances also shows a high correlation with the percentage of apoptotic cells in the sample (r2=0.965, P<0.003).
为了区分药物诱导的细胞死亡与因缺氧导致的自然细胞死亡，需要对凋亡和坏死进行鉴别。区分这两种细胞死亡模式的能力，尤其是在该过程的早期阶段，可能会对临床上评估抗癌药物治疗的结果产生重大影响。利用核磁共振波谱法来区分人宫颈癌（HeLa）细胞中的凋亡与坏死。通过用拓扑异构酶II抑制剂依托泊苷处理诱导凋亡，而通过使用依他尼酸或细胞松弛素B诱导坏死。我们发现，早在凋亡开始后6小时，亚甲基共振强度就会显著增加，但在坏死过程中不会发生这种变化。亚甲基与甲基共振的光谱强度比也与样品中凋亡细胞的百分比高度相关（r2 = 0.965，P < 0.003）。
