Folberg R, Chen X, Boldt H C, Pe'er J, Brown C K, Woolson R F, Maniotis A J
Department of Pathology, University of Illinois at Chicago, 1819 West Polk Street (446 CMW), Chicago, IL 60620, USA.
Ophthalmology. 2001 May;108(5):996-1001. doi: 10.1016/s0161-6420(01)00541-3.
To provide ophthalmologists and pathologists with expanded criteria for separating patients at high risk of metastatic melanoma from those at low risk on the basis of microcirculation patterns in choroidal and ciliary body melanomas.
Tissue culture studies and observational case series.
The pattern-forming ability of four uveal melanoma cell lines of varying degrees of aggressive behavior was studied in vitro. Histologic sections of 234 eyes removed for choroidal or ciliary body melanoma were studied for the presence of microcirculation patterns.
The study was divided into two phases: the study of histologic sections of eyes removed for choroidal and ciliary body melanomas and observations on the in vitro behavior of cultured melanoma cells of varying degrees of invasive behavior. The presence or absence of each of nine microcirculation patterns was recorded from tissue sections, and interrelationships between different patterns were explored statistically. In vitro reconstitution of patterns and a study of the interrelationships of patterns in histologic sections was carried out. In the in vitro studies, uveal melanoma cell lines of varying degrees of aggressive potential were cultured to observe the development of architectural patterns other than loops and networks.
In histologic studies, the outcome measure was the conditional probability of detecting loops or networks given the presence or absence of other patterns positive for periodic acid-SCHIFF: For tissue culture studies, the outcome measure was either the development or lack of development of patterns of different shapes in vitro.
Histologic studies disclosed that given the presence of arcs without or with branching in a tissue section, it is likely that loops or networks will be detected in the same section plane, suggesting that the production of these patterns by aggressive tumor cells reflects a spectrum of architectural potential. In vitro studies confirmed this hypothesis by revealing that highly aggressive and metastatic uveal melanoma cell lines, but not poorly aggressive tumor cell lines, generated parallel channels with and without crosslinking and arcs with and without branching as well as loops and networks.
The criteria for separating patients into low- and high-risk categories for metastasis from uveal melanoma should be expanded to include patterns other than loops or networks. In both the pathology laboratory as well as in a clinical setting, the detection of arcs or arcs with branching and parallel channels should prompt a careful search for loops and networks and for crosslinking parallel channels, respectively.
基于脉络膜和睫状体黑色素瘤的微循环模式，为眼科医生和病理学家提供更广泛的标准，以区分转移性黑色素瘤高风险患者和低风险患者。
组织培养研究和观察性病例系列。
在体外研究了四种具有不同侵袭性行为程度的葡萄膜黑色素瘤细胞系的模式形成能力。对因脉络膜或睫状体黑色素瘤而摘除的234只眼的组织切片进行研究，以确定是否存在微循环模式。
研究分为两个阶段：对因脉络膜和睫状体黑色素瘤而摘除的眼的组织切片进行研究，以及对不同侵袭性行为程度的培养黑色素瘤细胞的体外行为进行观察。从组织切片中记录九种微循环模式中每种模式的存在与否，并对不同模式之间的相互关系进行统计学探索。进行了模式的体外重建以及组织切片中模式相互关系的研究。在体外研究中，培养具有不同侵袭潜能的葡萄膜黑色素瘤细胞系，以观察除环和网络之外的结构模式的发展。
在组织学研究中，观察指标是在高碘酸-席夫染色阳性或阴性的其他模式存在或不存在的情况下，检测到环或网络的条件概率；对于组织培养研究，观察指标是体外不同形状模式的发展或未发展情况。
组织学研究表明，在组织切片中若存在无分支或有分支的弧，则很可能在同一切片平面中检测到环或网络，这表明侵袭性肿瘤细胞产生这些模式反映了一系列结构潜能。体外研究通过揭示高侵袭性和转移性葡萄膜黑色素瘤细胞系（而非低侵袭性肿瘤细胞系）产生有交联和无交联的平行通道以及有分支和无分支的弧以及环和网络，证实了这一假设。
将葡萄膜黑色素瘤患者分为转移低风险和高风险类别的标准应扩大，以包括环或网络以外的模式。在病理实验室和临床环境中，检测到有分支的弧或平行通道时，应分别仔细寻找环和网络以及交联的平行通道。
