Torres M, De Prost D, Hakim J, Gougerot M A
Eur J Clin Invest. 1979 Jun;9(3):209-17. doi: 10.1111/j.1365-2362.1979.tb00925.x.
The ingestion rate and oxygen-dependent metabolic activities of normal human polymorphonuclear leucocytes were measured with heat-killed Klebsiella as the particle. Since the experimental conditions were similar for each measurement, it was possible to make direct correlations between each oxygen-dependent reaction and (1) ingestion rate and (2) the other oxygen-dependent reactions. In the controls, oxygen-uptake was more reliably correlated (r = 0.960) with ingestion rates than with (in order of reliability) hydrogen peroxide produced (r = 0.860) and iodination (r = 0.858 and 0.813 for 100 and 20 micromol/l iodide respectively). Hydrogen peroxide production (r = 0.988), nitroblue tetrazolium reduction (r = 0.969) and cytochrome c reduction (r = 0.862) were more reliably correlated to oxygen-uptake than to ingestion rate, and iodination was better related to hydrogen peroxide production (r = 0.90 and 0.819 for 100 and 20 micromol/l iodide respectively) than to ingestion rate. From these findings it was possible to locate primary defects in abnormal polymorphonuclear leucocytes from individual patients with pyogenic infections, idiopathic refractory anaemia or idiopathic oesteomyelofibrosis with splenomegaly, even when several deficiencies existed.
以热灭活的克雷伯菌作为颗粒，测定正常人多形核白细胞的摄取率和氧依赖性代谢活性。由于每次测量的实验条件相似，因此可以将每个氧依赖性反应与（1）摄取率和（2）其他氧依赖性反应进行直接关联。在对照组中，与摄取率的相关性（r = 0.960）比与产生的过氧化氢（r = 0.860）以及碘化作用（分别为100和20 μmol/l碘化物时，r分别为0.858和0.813）的相关性更可靠（按可靠性排序）。过氧化氢的产生（r = 0.988）、硝基蓝四氮唑还原（r = 0.969）和细胞色素c还原（r = 0.862）与氧摄取的相关性比与摄取率的相关性更可靠，并且碘化作用与过氧化氢产生的相关性（分别为100和20 μmol/l碘化物时，r分别为0.90和0.819）比与摄取率的相关性更好。从这些发现中，即使存在多种缺陷，也有可能定位个别患有化脓性感染、特发性难治性贫血或伴有脾肿大的特发性骨髓纤维化的患者异常多形核白细胞中的原发性缺陷。
