Kim H M, Lee E H, Na H J, Lee S B, Shin T Y, Lyu Y S, Kim N S, Nomura S
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Chonbuk, South Korea.
J Ethnopharmacol. 1998 Jul;61(3):201-8. doi: 10.1016/s0378-8741(98)00040-3.
We investigated whether an aqueous extract of Polygala tenuifolia root (PTAE) inhibits secretion of tumor necrosis factor-alpha (TNF-alpha) from primary cultures of mouse astrocytes. PTAE dose-dependently inhibited the TNF-alpha secretion by astrocytes stimulated with substance P (SP) and lipopolysaccharide (LPS). Interleukin-1 (IL-1) has been shown to elevate TNF-alpha secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore also investigated whether IL-1 mediated inhibition of TNF-alpha secretion from primary astrocytes by PTAE. Treatment of PTAE to astrocytes stimulated with both LPS and SP decreased IL-1 secretion to the level observed with LPS alone. Moreover, incubation of astrocytes with IL-1 antibody abolished the synergistic co-operative effect of LPS and SP. These results suggest that PTAE may inhibit TNF-alpha secretion by inhibiting IL-1 secretion and that PTAE has an anti-inflammatory activity on the central nervous system curing some pathological disease states.
我们研究了远志根水提取物（PTAE）是否能抑制小鼠星形胶质细胞原代培养物中肿瘤坏死因子-α（TNF-α）的分泌。PTAE剂量依赖性地抑制了由P物质（SP）和脂多糖（LPS）刺激的星形胶质细胞分泌TNF-α。白细胞介素-1（IL-1）已被证明能提高LPS刺激的星形胶质细胞中TNF-α的分泌，而在无LPS的情况下对星形胶质细胞没有影响。因此，我们还研究了IL-1是否介导了PTAE对原代星形胶质细胞分泌TNF-α的抑制作用。用PTAE处理LPS和SP共同刺激的星形胶质细胞，可使IL-1分泌降至仅用LPS刺激时观察到的水平。此外，用IL-1抗体孵育星形胶质细胞可消除LPS和SP的协同合作效应。这些结果表明，PTAE可能通过抑制IL-1分泌来抑制TNF-α分泌，并且PTAE在治疗某些病理疾病状态时对中枢神经系统具有抗炎活性。
