新生儿和成人中乳酸酸中毒对补体系统的体外激活作用。
In-vitro activation of complement system by lactic acidosis in newborn and adults.
作者信息
Hecke F, Hoehn T, Strauss E, Obladen M, Sonntag J
机构信息
Department of Neonatology, Charité Virchow Hospital, Humboldt University, Berlin, Germany.
出版信息
Mediators Inflamm. 2001 Feb;10(1):27-31. doi: 10.1080/09629350123788.
INTRODUCTION
Complement activation occurs secondary to a variety of external stimuli. Lactic acidosis has been previously shown to activate the complement factors C3a and C5a. In the present investigation we examined the differential effect of lactic acidosis on anaphylatoxin levels in cord and adult blood. Furthermore we aimed to determine if the entire complement cascade could be activated by lactic acidosis.
METHODS
Cord and adult blood samples (n = 20 each) were collected and incubated for one hour in either untreated condition or with the addition of lactate in two concentrations (5.5 mmol/l vs. 22 mmol/l). Following incubation, levels of C3a, C5a and sC5b-9, and blood gas parameters were determined.
RESULTS
Anaphylatoxin (C3a and C5a) and sC5b-9 levels increased with the addition of lactate in a dose-dependent manner in cord and adult blood (C3a: 1 h, 5.5 mmo/l, 22 mmol/l: 418/498/622 microg/l in cord blood; 1010/1056/1381 microg/l in adult blood, p<0,05; similar results were found for C5a and sC5b-9).
CONCLUSION
Lactic acidosis leads to an activation of the entire complement system in neonates and in adults. This activation is dose-dependent and more pronounced in adults as compared to neonates.
引言
补体激活继发于多种外部刺激。先前已证明乳酸性酸中毒可激活补体因子C3a和C5a。在本研究中，我们检测了乳酸性酸中毒对脐带血和成人血液中过敏毒素水平的不同影响。此外，我们旨在确定乳酸性酸中毒是否能激活整个补体级联反应。
方法
收集脐带血和成人血液样本（各n = 20），在未处理条件下或添加两种浓度乳酸（5.5 mmol/l与22 mmol/l）的情况下孵育1小时。孵育后，测定C3a、C5a和sC5b-9的水平以及血气参数。
结果
在脐带血和成人血液中，随着乳酸的添加，过敏毒素（C3a和C5a）和sC5b-9水平呈剂量依赖性增加（C3a：1小时，5.5 mmol/l、22 mmol/l：脐带血中分别为418/498/622 μg/l；成人血液中为1010/1056/1381 μg/l，p<0.05；C5a和sC5b-9也有类似结果）。
结论
乳酸性酸中毒导致新生儿和成人的整个补体系统激活。这种激活是剂量依赖性的，与新生儿相比，在成人中更明显。
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