利用原子力显微镜研究DNA的表面生物学

Surface biology of DNA by atomic force microscopy.

作者信息

Hansma H G

机构信息

Department of Physics, University of California, Santa Barbara, California 93106, USA.

出版信息

Annu Rev Phys Chem. 2001;52:71-92. doi: 10.1146/annurev.physchem.52.1.71.

DOI:10.1146/annurev.physchem.52.1.71

PMID:11326059

Abstract

The atomic force microscope operates on surfaces. Since surfaces occupy much of the space in living organisms, surface biology is a valid and valuable form of biology that has been difficult to investigate in the past owing to a lack of good technology. Atomic force microscopy (AFM) of DNA has been used to investigate DNA condensation for gene therapy, DNA mapping and sizing, and a few applications to cancer research and to nanotechnology. Some of the most exciting new applications for atomic force microscopy of DNA involve pulling on single DNA molecules to obtain measurements of single-molecule mechanics and thermodynamics.

摘要

原子力显微镜在表面上运行。由于表面占据了生物体中的大部分空间，表面生物学是一种合理且有价值的生物学形式，过去由于缺乏良好的技术而难以进行研究。DNA的原子力显微镜（AFM）已被用于研究用于基因治疗的DNA凝聚、DNA图谱绘制和大小测定，以及在癌症研究和纳米技术中的一些应用。DNA原子力显微镜一些最令人兴奋的新应用涉及拉伸单个DNA分子以获得单分子力学和热力学的测量值。