London Centre for Nanotechnology, University College London, London, UK.
Molecular Sciences Research Hub, Department of Chemistry, Imperial College London, London, UK.
Methods Mol Biol. 2022;2476:43-62. doi: 10.1007/978-1-0716-2221-6_5.
Atomic force microscopy (AFM) is a microscopy technique that uses a sharp probe to trace a sample surface at nanometer resolution. For biological applications, one of its key advantages is its ability to visualize the substructure of single molecules and molecular complexes in an aqueous environment. Here, we describe the application of AFM to determine the secondary and tertiary structure of surface-bound DNA, and its interactions with proteins.
原子力显微镜（AFM）是一种使用探针在纳米分辨率下追踪样品表面的显微镜技术。对于生物应用来说，其关键优势之一是能够在水相环境中可视化单分子和分子复合物的亚结构。本文介绍了将 AFM 应用于确定表面结合 DNA 的二级和三级结构及其与蛋白质相互作用的方法。
