Dal-Pizzol F, Klamt F, Benfato M S, Bernard E A, Moreira J C
Laboratório de Estresse Oxidativo, Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Free Radic Res. 2001 Apr;34(4):395-404. doi: 10.1080/10715760100300331.
Recent intervention studies revealed that supplementation with retinoids resulted in a higher incidence of lung cancer. Recently the causal mechanism has begun to be clarified. We report here that retinol caused cellular oxidative stress and modulated superoxide dismutase, catalase and glutathione peroxidase activities. Retinol (7 microM) significantly increased TBARS, conjugated dienes, and hydroperoxide-initiated chemiluminescence in cultured Sertoli cells. In response to retinol treatment superoxide dismutase, catalase and glutathione peroxidase activities increased. TBARS content and catalase activities were decreased by a free radical scavenger. These findings suggest that retinol may induce oxidative stress and modulate antioxidant enzyme activities in Sertoli cells.
近期的干预研究表明，补充视黄醇会导致肺癌发病率升高。最近，其因果机制已开始得到阐明。我们在此报告，视黄醇会引起细胞氧化应激，并调节超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。视黄醇（7微摩尔）显著增加了培养的支持细胞中的硫代巴比妥酸反应物（TBARS）、共轭二烯和氢过氧化物引发的化学发光。在视黄醇处理后，超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性增加。自由基清除剂可降低TBARS含量和过氧化氢酶活性。这些发现表明，视黄醇可能诱导支持细胞中的氧化应激并调节抗氧化酶活性。
