Metabolite profiling and adaptation mechanisms of under pH stress.

INTRODUCTION

pH is an important environmental factor affecting the survival of fungi, and , which can grow and reproduce over a wide range of pH, is suitable for studying their adaptation mechanism to pH stress.

METHODS

In this study, was cultured on plates of different initial pH (pH 3.8-8.0), with the results revealing distinct morphologies at pH 3.0-5.0, pH 6.0-7.0 and pH 8.0. Liquid chromatography-mass spectrometry (LC-MS) and multivariate analysis subsequently were used to analyze the changes of substance metabolism of at different pH.

RESULTS AND DISCUSSION

LC-MS and multivariate analyses showed that 's growth at different pH involved significantly different metabolites. In particular, comparing pH 4.0 vs pH 6.0, pH 6.0 vs pH 8.0 and pH 4.0 vs pH 8.0 revealed a total of 317, 171 and 404 significantly different substances, respectively. Finally, as the pH changed from 4.0 to 6.0 to 8.0, eight changes in the patterns of differential substances were identified. At low pH, accumulated large amounts of energy substances (e.g., adenosine), active antioxidants (e.g., glutathione) and osmo-protective substances (e.g., raffinose). In contrast, at high pH, large amounts of phosphatidylcholine (PC), lysophosphatidyl ethanolamine (LPE), lysophosphatidyl choline (LPC), lysophosphatidyl serine (LPS) related to biofilms were synthesized, alongside antioxidants (e.g., formononetin) and acidic substances. The aforementioned results indicate that adapts to changes in pH by adjusting their metabolite synthesis. Therefore, under unsuitable pH environments, synthesizes specific sets of metabolites that play key roles to cope with the stress.