Sinpru Panpradub, Riou Cindy, Kubota Satoshi, Poompramun Chotima, Molee Wittawat, Molee Amonrat
School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Animals (Basel). 2021 Sep 5;11(9):2606. doi: 10.3390/ani11092606.
Improving feed efficiency is an important breeding target for the poultry industry; to achieve this, it is necessary to understand the molecular basis of feed efficiency. We compared the jejunal transcriptomes of low- and high-feed conversion ratio (FCR) slow-growing Korat chickens (KRs). Using an original sample of 75 isolated 10-week-old KR males, we took jejunal samples from six individuals in two groups: those with extremely low FCR ( = 3; FCR = 1.93 ± 0.05) and those with extremely high FCR ( = 3; FCR = 3.29 ± 0.06). Jejunal transcriptome profiling via RNA sequencing revealed 56 genes that were differentially expressed ( < 0.01, FC > 2): 31 were upregulated, and 25 were downregulated, in the low-FCR group relative to the high-FCR group. Functional annotation revealed that these differentially expressed genes were enriched in biological processes related to immune response, glutathione metabolism, vitamin transport and metabolism, lipid metabolism, and neuronal and cardiac maturation, development, and growth, suggesting that these are important mechanisms governing jejunal feed conversion. These findings provide an important molecular basis for future breeding strategies to improve slow-growing chicken feed efficiency.
提高饲料效率是家禽业的一个重要育种目标；要实现这一目标，有必要了解饲料效率的分子基础。我们比较了低饲料转化率（FCR）和高饲料转化率的慢速生长的呵叻鸡（KRs）的空肠转录组。我们从75只10周龄的KR雄性个体中选取原始样本，将两组中各6只个体的空肠样本进行分析：极低FCR组（n = 3；FCR = 1.93±0.05）和极高FCR组（n = 3；FCR = 3.29±0.06）。通过RNA测序进行空肠转录组分析，结果显示有56个基因存在差异表达（P < 0.01，FC > 2）：相对于高FCR组，低FCR组中有31个基因上调，25个基因下调。功能注释表明，这些差异表达基因在与免疫反应、谷胱甘肽代谢、维生素转运和代谢、脂质代谢以及神经元和心脏成熟、发育和生长相关的生物学过程中富集，这表明这些是控制空肠饲料转化的重要机制。这些发现为未来提高慢速生长鸡饲料效率的育种策略提供了重要的分子基础。
