Saeki K, Takahashi Y, Oh-Oka H, Umeoka T, Oda Y, Fukuyama K
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Japan.
Biosci Biotechnol Biochem. 2001 Mar;65(3):719-24. doi: 10.1271/bbb.65.719.
The amino acid sequence of the coat protein (CP) of a tobacco necrosis virus (TNV) strain, Toyama isolate, was determined by a combination of peptide and cDNA sequencing. The deduced sequence of 276 residues was compared with CPs of other TNV isolates and other plant virus isolates of Tombusviridae. It showed the highest similarity to the TNV Nebraska isolate with 92% identity and moderate similarity to the TNV strain A with 51% identity, confirming the previous serological analysis. It also showed overall similarity with CPs of mostly genera Necrovirus and Sobemovirus, and partial similarity with CPs of genera Tombusvirus and Carmovirus. Among 13 CPs that showed overall similarity, there were 10 completely conserved residues. These included three residues that participate in Ca2+ ligation at the interfaces of virion subunits in TNV crystal structure, suggesting that similar metal binding occur in the viruses of genera Necrovirus and Sobemovirus.
通过肽段测序和cDNA测序相结合的方法，确定了烟草坏死病毒（TNV）富山分离株外壳蛋白（CP）的氨基酸序列。将推导得到的276个残基的序列与其他TNV分离株以及番茄病毒科其他植物病毒分离株的CP进行了比较。结果显示，它与TNV内布拉斯加分离株的相似性最高，同一性为92%，与TNV A株的相似性中等，同一性为51%，这证实了之前的血清学分析。它还与大多数坏死病毒属和南方菜豆花叶病毒属的CP具有总体相似性，与番茄病毒属和 Carmovirus属的CP具有部分相似性。在13个显示出总体相似性的CP中，有10个完全保守的残基。其中包括在TNV晶体结构中病毒粒子亚基界面处参与Ca2+连接作用的三个残基，这表明在坏死病毒属和南方菜豆花叶病毒属的病毒中可能发生类似的金属结合。
