小鼠组织和细胞系中表皮型转谷氨酰胺酶（TGase 3）表达的分析。

Analysis of epidermal-type transglutaminase (TGase 3) expression in mouse tissues and cell lines.

作者信息

Hitomi K, Horio Y, Ikura K, Yamanishi K, Maki M

机构信息

Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, 464-8601, Nagoya, Japan.

出版信息

Int J Biochem Cell Biol. 2001 May;33(5):491-8. doi: 10.1016/s1357-2725(01)00033-4.

DOI:10.1016/s1357-2725(01)00033-4

PMID:11331204

Abstract

In the formation of the cornified cell envelope in the epidermis, epidermal-type transglutaminase (TGase 3) cross-links a variety of structural proteins. However, its expression in other tissue has not been investigated. Furthermore, no cell line expressing TGase 3 has been found. The tissue distribution of TGase 3 in mice was investigated using reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting analyses. TGase 3 mRNA was expressed in the brain, stomach, spleen, small intestine, testis, skeletal muscle and skin. The stomach and testis expressed TGase 3 protein in size similar to that observed in the epidermis. Screening various cell lines, a gastric human cancer cell line, MKN-1 and mouse neuroblast cell line, neuro2a, were found to express TGase 3.

摘要

在表皮角质化细胞包膜的形成过程中，表皮型转谷氨酰胺酶（TGase 3）使多种结构蛋白发生交联。然而，其在其他组织中的表达尚未得到研究。此外，尚未发现表达TGase 3的细胞系。利用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析研究了TGase 3在小鼠体内的组织分布。TGase 3 mRNA在脑、胃、脾、小肠、睾丸、骨骼肌和皮肤中表达。胃和睾丸中表达的TGase 3蛋白大小与在表皮中观察到的相似。通过筛选各种细胞系，发现一种人胃癌细胞系MKN-1和小鼠神经母细胞瘤细胞系neuro2a表达TGase 3。