Flåøyen A, Wilkins A L, Deng D, Brekke T
National Veterinary Institute, Oslo, Norway.
Vet Res Commun. 2001 Apr;25(3):225-38. doi: 10.1023/a:1006485726523.
A sheep was dosed three times per day over six consecutive days with 70 g Narthecium ossifragum, and once on the seventh day with 70 g N. ossifragum. Additionally, it was dosed once on days 1-7 with 20 mg of [20,23,23-2H3]sarsasapogenin. After 7 days, the sheep was killed and GC-MS analysis of the free and conjugated sapogenin content in bile, urine, rumen, duodenum, jejunum, colon and rectum samples collected from the sheep, faecal samples collected on days 4-7, and dosed plant material was performed. The N. ossifragum contained mainly sarsasapogenin and smilagenin. Only neglible levels of deuterium-labelled sarsasapogenins were detected in the samples from the animal. Ingested saponins were quickly hydrolysed in the rumen to free sapogenins and, in part, epimerized at C-3 to afford episapogenins. The absorption of free sapogenins appeared to occur in the jejunum. The concentration of sapogenins in faeces reached a plateau 108 h after dosing started.
一只羊连续六天每天服用三次70克骨碎补，第七天服用一次70克骨碎补。此外，在第1至7天每天给它服用一次20毫克的[20,23,23-2H3]菝葜皂苷元。7天后，将羊处死，并对从羊身上采集的胆汁、尿液、瘤胃、十二指肠、空肠、结肠和直肠样本、第4至7天采集的粪便样本以及给药的植物材料中的游离和结合皂苷元含量进行气相色谱-质谱分析。骨碎补中主要含有菝葜皂苷元和薯蓣皂苷元。在动物样本中仅检测到极低水平的氘标记菝葜皂苷元。摄入的皂苷在瘤胃中迅速水解为游离皂苷元，部分在C-3位发生差向异构化生成表皂苷元。游离皂苷元的吸收似乎发生在空肠。给药开始后108小时，粪便中皂苷元的浓度达到稳定水平。
