Holland W G, Claes F, My L N, Thanh N G, Tam P T, Verloo D, Büscher P, Goddeeris B, Vercruysse J
Faculty of Veterinary Medicine, Laboratory of Parasitology, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.
Vet Parasitol. 2001 May 9;97(1):23-33. doi: 10.1016/s0304-4017(01)00381-8.
In this study five parasitological methods and a polymerase chain reaction (PCR) were compared for the diagnostic sensitivity for Trypanosoma evansi in experimentally infected water buffaloes over a period of 15 weeks. The combined estimates of sensitivity (CE(se)) of the PCR proved to be highest at 78.2%, closely followed by the mouse inoculation (MI), the micro-haematocrite centrifugation technique (MHCT) and the mini-anion-exchange centrifugation technique (MAECT) with CE(se) of, respectively, 74.0, 69.6 and 62.4%. The CE(se) of the buffy-coat technique (BCT) at 38.6% and the sodium dodecyl sulfate (SDS) clarification technique at 25.1% were considerably lower. PCR detected consistently all buffaloes infected from week 3 post-infection (PI) onwards. For MI this occurred after 5 weeks PI while for MHCT and MAECT these sustainable high levels were reached in the 7th week PI. BCT and SDS never detected all buffaloes infected. The influence of time and temperature on the viability of T. evansi in heparinized blood from water buffalo was also studied. In general we observed that the survival time tends to be longer when blood is kept at 4 degrees C. In samples kept in direct sunlight parasites became undetectable with the MHCT after 30min. After treatment of the water buffaloes with diminazene aceturate, the PCR signal disappeared within 24h.
在本研究中，对五种寄生虫学方法和一种聚合酶链反应（PCR）在15周内对实验感染水牛体内伊氏锥虫的诊断敏感性进行了比较。PCR的综合敏感性估计值（CE(se)）最高，为78.2%，紧随其后的是小鼠接种法（MI）、微量血细胞比容离心技术（MHCT）和微量阴离子交换离心技术（MAECT），其CE(se)分别为74.0%、69.6%和62.4%。血沉棕黄层技术（BCT）的CE(se)为38.6%，十二烷基硫酸钠（SDS）澄清技术的CE(se)为25.1%，明显更低。PCR从感染后（PI）第3周起始终能检测到所有感染的水牛。对于MI，在感染后5周出现这种情况，而对于MHCT和MAECT，在感染后第7周达到这些持续的高水平。BCT和SDS从未检测到所有感染的水牛。还研究了时间和温度对水牛肝素化血液中伊氏锥虫活力的影响。总体而言，我们观察到血液保存在4℃时存活时间往往更长。在直接阳光下保存的样本中，30分钟后用MHCT就检测不到寄生虫了。用水杨酸乙酰苯胺治疗水牛后，PCR信号在24小时内消失。
