Silverman M, Simon M
J Bacteriol. 1973 Jan;113(1):105-13. doi: 10.1128/jb.113.1.105-113.1973.
Flagellar mutants in Escherichia coli were obtained by selection for resistance to the flagellotropic phage chi. F elements covering various regions of the E. coli genome were then constructed, and, on the basis of the ability of these elements to restore flagellar function, the mutations were assigned to three regions of the E. coli chromosome. Region I is between trp and gal; region II is between uvrC and aroD; and region III is between his and uvrC. F elements carrying flagellar mutations were constructed. Stable merodiploid strains with a flagellar defect on the exogenote and another on the endogenote were then prepared. These merodiploids yielded information on the complementation behavior of mutations in a given region. Region III was shown to include at least six cistrons, A, B, C, D, E, and F. Region II was shown to include at least four cistrons, G, H, I, and J. Examination of the phenotypes of the mutants revealed that those with lesions in cistron E of region III produce "polyhooks" and lesions in cistron F of region III result in loss of ability to produce flagellin. Mutants with lesions in cistron J of region II were entirely paralyzed (mot) mutants. Genetic analysis of flagellar mutations in region III suggested that the mutations located in cistrons A, B, C, and E are closely linked and mutations in cistrons D and F are closely linked.
通过筛选对嗜鞭毛噬菌体chi具有抗性的菌株，获得了大肠杆菌的鞭毛突变体。然后构建了覆盖大肠杆菌基因组各个区域的F因子，并根据这些因子恢复鞭毛功能的能力，将突变定位到大肠杆菌染色体的三个区域。区域I在trp和gal之间；区域II在uvrC和aroD之间；区域III在his和uvrC之间。构建了携带鞭毛突变的F因子。接着制备了在外源染色体和内源染色体上均存在鞭毛缺陷的稳定部分二倍体菌株。这些部分二倍体提供了关于给定区域内突变互补行为的信息。结果表明区域III至少包含六个顺反子，即A、B、C、D、E和F。区域II至少包含四个顺反子，即G、H、I和J。对突变体表型的检查发现，区域III中顺反子E发生损伤的突变体产生“多钩”，区域III中顺反子F发生损伤的突变体导致失去产生鞭毛蛋白的能力。区域II中顺反子J发生损伤的突变体完全是麻痹型（mot）突变体。对区域III中鞭毛突变的遗传分析表明，位于顺反子A、B、C和E中的突变紧密连锁，位于顺反子D和F中的突变紧密连锁。
