Bucher V, Brunner B, Leibrock C, Schubert M, Nisch W
Natural and Medical Sciences Institute, Markwiesenstrasse 55, D-72770, Reutlingen, Germany.
Biosens Bioelectron. 2001 May;16(3):205-10. doi: 10.1016/s0956-5663(01)00135-x.
A light-addressable microelectrode chip with 3600 TiN electrodes was fabricated. Amorphous silicon (a-Si:H) serves as a photo conductor. The electrodes on the chip are addressed by a laser spot and electrical properties of the system are determined. DC measurements show a dark to bright dynamic of 10(6)-10(7). The AC impedance dynamic @ 1 kHz/100 mV and thus the signal-to-noise-ratio is determined to 60. This value is quite sufficient for electrophysiological measurements. For the first time, recordings from cardiac myocytes are reported using the principle of light-addressing. Measurements were done with a standard laser scan microscope (Zeiss LSM 410).
制造了一种带有3600个氮化钛电极的光寻址微电极芯片。非晶硅（a-Si:H）用作光电导体。芯片上的电极由激光光斑寻址，并测定系统的电学性质。直流测量显示暗态到亮态的动态范围为10(6)-10(7)。在1 kHz/100 mV下测定的交流阻抗动态范围，进而确定信噪比为60。该值对于电生理测量来说已经足够。首次报道了利用光寻址原理对心肌细胞进行记录。测量是使用标准激光扫描显微镜（蔡司LSM 410）进行的。
