Connolly P, Clark P, Curtis A S, Dow J A, Wilkinson C D
Department of Electronics and Electrical Engineering, University of Glasgow, UK.
Biosens Bioelectron. 1990;5(3):223-34. doi: 10.1016/0956-5663(90)80011-2.
This paper describes a planar array of microelectrodes developed for monitoring the electrical activity of cells in culture. The device allows the incorporation of surface topographical features in an insulating layer above the electrodes. Semiconductor technology is employed for the fabrication of the gold electrodes and for the deposition and patterning of an insulating layer of silicon nitride. The electrodes have been tested using a cardiac cell culture of chick embryo myocytes, and the physical beating of the cultured cells correlated with the simultaneous extracellular voltage measurements obtained. It was found that extracellular stimulation of the cells was possible via the same electrodes used for recording.
本文描述了一种为监测培养细胞的电活动而开发的平面微电极阵列。该装置允许在电极上方的绝缘层中引入表面形貌特征。采用半导体技术制造金电极以及沉积和图案化氮化硅绝缘层。已使用鸡胚心肌细胞的心脏细胞培养物对电极进行了测试，并且培养细胞的物理跳动与同时获得的细胞外电压测量结果相关。结果发现，通过用于记录的相同电极对细胞进行细胞外刺激是可行的。
