固定化红平红球菌KA2-5-1细胞的长期重复生物脱硫
Long-term repeated biodesulfurization by immobilized Rhodococcus erythropolis KA2-5-1 cells.
作者信息
Naito M, Kawamoto T, Fujino K, Kobayashi M, Maruhashi K, Tanaka A
机构信息
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Japan.
出版信息
Appl Microbiol Biotechnol. 2001 Apr;55(3):374-8. doi: 10.1007/s002530000527.
In this study, biodesulfurization (BDS) was carried out using immobilized Rhodococcus erythropolis KA2-5-1 in n-tetradecane containing dibenzothiophene (DBT) as a model oil (n-tetradecane/immobilized cell biphasic system). The cells were immobilized by entrapping them with calcium alginate, agar, photo-crosslinkable resin prepolymers (ENT-4000 and ENTP-4000), and urethane prepolymers (PU-3 and PU-6); and it was found that ENT-4000-immobilized cells had the highest DBT desulfurization activity in the model oil system without leakage of cells from the support. Furthermore, ENT4000-immobilized cells could catalyze BDS repeatedly in this system for more than 900 h with reactivation; and recovery of both the biocatalyst and the desulfurized model oil was easy. This study would give a solution to the problems in BDS, such as the troublesome process of recovering desulfurized oil and the short life of BDS biocatalysts.
在本研究中，使用固定化的红平红球菌KA2-5-1在含有二苯并噻吩（DBT）的正十四烷作为模型油（正十四烷/固定化细胞双相体系）中进行生物脱硫（BDS）。通过用海藻酸钙、琼脂、光可交联树脂预聚物（ENT-4000和ENTP-4000）和聚氨酯预聚物（PU-3和PU-6）包埋细胞来固定细胞；并且发现，在模型油体系中，ENT-4000固定化细胞具有最高的DBT脱硫活性，且细胞不会从载体上泄漏。此外，ENT4000固定化细胞在该体系中经再活化后可重复催化BDS超过900小时；生物催化剂和脱硫模型油的回收都很容易。本研究将为BDS中存在的问题提供解决方案，例如回收脱硫油的麻烦过程以及BDS生物催化剂寿命短的问题。
Zotero 插件