用包覆有磁铁矿纳米颗粒的微生物细胞对二苯并噻吩进行生物脱硫
Biodesulfurization of dibenzothiophene by microbial cells coated with magnetite nanoparticles.
作者信息
Shan GuoBin, Xing JianMin, Zhang HuaiYing, Liu HuiZhou
机构信息
Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box No. 353, Beijing, 100080 China.
出版信息
Appl Environ Microbiol. 2005 Aug;71(8):4497-502. doi: 10.1128/AEM.71.8.4497-4502.2005.
Microbial cells of Pseudomonas delafieldii were coated with magnetic Fe3O4 nanoparticles and then immobilized by external application of a magnetic field. Magnetic Fe3O4 nanoparticles were synthesized by a coprecipitation method followed by modification with ammonium oleate. The surface-modified Fe3O4 nanoparticles were monodispersed in an aqueous solution and did not precipitate in over 18 months. Using transmission electron microscopy (TEM), the average size of the magnetic particles was found to be in the range from 10 to 15 nm. TEM cross section analysis of the cells showed further that the Fe3O4 nanoparticles were for the most part strongly absorbed by the surfaces of the cells and coated the cells. The coated cells had distinct superparamagnetic properties. The magnetization (delta(s)) was 8.39 emu.g(-1). The coated cells not only had the same desulfurizing activity as free cells but could also be reused more than five times. Compared to cells immobilized on Celite, the cells coated with Fe3O4 nanoparticles had greater desulfurizing activity and operational stability.
将德氏假单胞菌的微生物细胞用磁性Fe3O4纳米颗粒包覆，然后通过施加外部磁场进行固定。磁性Fe3O4纳米颗粒通过共沉淀法合成，随后用油酸铵进行改性。表面改性的Fe3O4纳米颗粒在水溶液中呈单分散状态，在超过18个月的时间里都没有沉淀。使用透射电子显微镜（TEM）发现，磁性颗粒的平均尺寸在10到15纳米范围内。对细胞的TEM横截面分析进一步表明，Fe3O4纳米颗粒大部分被细胞表面强烈吸附并包覆在细胞上。包覆后的细胞具有明显的超顺磁性。磁化强度（δ(s)）为8.39 emu·g(-1)。包覆后的细胞不仅具有与游离细胞相同的脱硫活性，而且还可以重复使用超过五次。与固定在硅藻土上的细胞相比，包覆有Fe3O4纳米颗粒的细胞具有更高的脱硫活性和操作稳定性。
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