Li Y G, Xing J M, Xiong X C, Li W L, Gao H S, Liu H Z
Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
J Ind Microbiol Biotechnol. 2008 Mar;35(3):145-50. doi: 10.1007/s10295-007-0268-7. Epub 2007 Nov 6.
The immobilization of Pseudomonas delafieldii R-8 in calcium alginate beads has been studied in order to improve biodesulfurization activity in oil/water (O/W) biphasic systems. A gas jet extrusion technique was performed to produce immobilized beads. The specific desulfurization rate of 1.5 mm diameter beads was 1.4-fold higher than that of 4.0 mm. Some nonionic surfactants can significantly increase the activity of immobilized cells. The desulfurization rate with the addition of 0.5% Span 80 increased 1.8-fold compared with that of the untreated beads. The rate of biodesulfurization was markedly enhanced by decreasing the size of alginate beads and adding the surfactant Span 80, most likely resulting from the increasing mass transfer of substrate to gel matrix.
为提高在油/水（O/W）双相体系中的生物脱硫活性，对德氏假单胞菌R-8固定于海藻酸钙珠粒中的情况进行了研究。采用气体喷射挤压技术制备固定化珠粒。直径1.5毫米珠粒的比脱硫率比4.0毫米的高1.4倍。一些非离子表面活性剂可显著提高固定化细胞的活性。添加0.5%司盘80时的脱硫率比未处理珠粒提高了1.8倍。通过减小海藻酸钙珠粒尺寸并添加表面活性剂司盘80，生物脱硫速率显著提高，最可能的原因是底物向凝胶基质的传质增加。
