Hsiao G, Teng C M, Sheu J R, Cheng Y W, Lam K K, Lee Y M, Wu T S, Yen M H
Department of Pharmacology, Taipei Medical Univeristy, Taiwan.
Biochim Biophys Acta. 2001 Feb 16;1525(1-2):77-88. doi: 10.1016/s0304-4165(00)00173-2.
The antioxidant properties of cinnamophilin were evaluated by studying its ability to react with relevant reactive oxygen species, and its protective effect on cultured cells and biomacromolecules under oxidative stress. Cinnamophilin concentration-dependently suppressed non-enzymatic iron-induced lipid peroxidation in rat brain homogenates with an IC50 value of 8.0+/-0.7 microM and iron ion/ADP/ascorbate-initiated rat liver mitochondrial lipid peroxidation with an IC50 value of 17.7+/-0.2 microM. It also exerted an inhibitory activity on NADPH-dependent microsomal lipid peroxidation with an IC50 value of 3.4+/-0.1 microM without affecting microsomal electron transport of NADPH-cytochrome P-450 reductase. Both 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azo-bis(2-amidinopropane) dihydrochloride-derived peroxyl radical tests demonstrated that cinnamophilin possessed marked free radical scavenging capacity. Cinnamophilin significantly protected cultured rat aortic smooth muscle cells (A7r5) against alloxan/iron ion/H2O2-induced damage resulting in cytoplasmic membranous disturbance and mitochondrial potential decay. By the way, cinnamophilin inhibited copper-catalyzed oxidation of human low-density lipoprotein, as measured by fluorescence intensity and thiobarbituric acid-reactive substance formation in a concentration-dependent manner. On the other hand, it was reactive toward superoxide anions generated by the xanthine/xanthine oxidase system and the aortic segment from aged spontaneously hypertensive rat. Furthermore, cinnamophilin exerted a divergent effect on the respiratory burst of human neutrophil by different stimulators. Our results show that cinnamophilin acts as a novel antioxidant and cytoprotectant against oxidative damage.
通过研究肉桂亲和素与相关活性氧的反应能力及其在氧化应激下对培养细胞和生物大分子的保护作用，评估了其抗氧化特性。肉桂亲和素浓度依赖性地抑制大鼠脑匀浆中由非酶铁诱导的脂质过氧化，IC50值为8.0±0.7微摩尔，以及铁离子/ADP/抗坏血酸引发的大鼠肝线粒体脂质过氧化，IC50值为17.7±0.2微摩尔。它还对NADPH依赖性微粒体脂质过氧化具有抑制活性，IC50值为3.4±0.1微摩尔，且不影响微粒体中NADPH-细胞色素P-450还原酶的电子传递。1,1-二苯基-2-苦基肼和2,2'-偶氮双(2-脒基丙烷)二盐酸盐衍生的过氧自由基试验均表明，肉桂亲和素具有显著的自由基清除能力。肉桂亲和素显著保护培养的大鼠主动脉平滑肌细胞(A7r5)免受四氧嘧啶/铁离子/H2O2诱导的损伤，导致细胞质膜紊乱和线粒体电位衰减。顺便提一下，肉桂亲和素以浓度依赖性方式抑制铜催化的人低密度脂蛋白氧化，通过荧光强度和硫代巴比妥酸反应性物质的形成来测定。另一方面，它对黄嘌呤/黄嘌呤氧化酶系统产生的超氧阴离子以及老年自发性高血压大鼠的主动脉段有反应。此外，肉桂亲和素对不同刺激剂诱导的人中性粒细胞呼吸爆发有不同影响。我们的结果表明，肉桂亲和素作为一种新型抗氧化剂和细胞保护剂，可抵抗氧化损伤。
