Devi R Bhuvanamha, A R Subhashree, Parameaswari P J, Parijatham B O
Post Graduate, Department of Pathology, Sree Balaji Medical College & Hospital , Chrompet, Chennai, Tamil nadu, India .
J Clin Diagn Res. 2013 May;7(5):835-9. doi: 10.7860/JCDR/2013/5630.2953. Epub 2013 Mar 29.
Microwave irradiation has been tried as a replacement for the conventional tissue processing technique in histopathology laboratories for quite some time. Studies have shown that Domestic Microwave Tissue Processing (DMWTP) provides a faster delivery of the tissue sections with a morphology which is similar to that which is seen Conventional Tissue Processing (CTP). But many laboratories still confine the domestic microwave tissue processing method only to the handle selected specimens, for which urgent reports are needed. One of the probable reasons is that, understanding about the number of tissue sections which can be processed using a microwave oven at a time, with the appropriate quality, still remains unclear.
The aim of this study was to quantitatively analyze the optimum number of samples that a domestic microwave could process at a time, as well as to qualitatively analyze the morphological outcome of those tissue sections with that of conventional processing.
This study was approved by the research and ethical committee of Sree Balaji Medical College and Hospital. A total of 135 paired tissue sections were included in the study. Ten tissue sections (which are mentioned hereafter as A10) were processed in a domestic microwave and their paired 10 tissues were processed by a conventional method. Subsequently, the number of tissues which was to be processed was increased to B15, C20, D25, E30 and F35, after ascertaining that the morphological qualities of the previously processed tissue sections were satisfactory. Sections of 4 μm thickness were taken and they were stained by the Haematoxylin and Eosin method. The slides of the tissues which were processed by the microwave method and the conventional method were randomly numbered, for a blind study, which were independently evaluated by two observers. The qualities of slides were assessed, based on 4 parameters: the cytoplasmic details, the nuclear details, the tissue architecture and the staining characteristics. The statistical analysis was done by using SPSS 15.0.
The morphological outcomes (quality) of the DMWTPs were comparable to that of the CTPs, when the sample load (quantity) in the microwave oven was up to 25 samples.
Domestic microwave processing can be effectively used in laboratories with a maximum sample size of 25 samples per load. This has the advantage of being rapid, with its morphological quality being identical to that of conventional processing.
一段时间以来，微波辐照已被尝试作为组织病理学实验室中传统组织处理技术的替代方法。研究表明，家用微波组织处理（DMWTP）能更快地提供形态与传统组织处理（CTP）相似的组织切片。但许多实验室仍仅将家用微波组织处理方法局限于处理急需报告的选定标本。可能的原因之一是，对于一次使用微波炉能处理的具有适当质量的组织切片数量，仍不清楚。
本研究的目的是定量分析家用微波炉一次能处理的最佳样本数量，并定性分析这些组织切片与传统处理后的形态学结果。
本研究经斯里·巴拉吉医学院和医院研究与伦理委员会批准。共纳入135对组织切片用于研究。10个组织切片（以下简称A10）采用家用微波处理，其配对的10个组织采用传统方法处理。在确定先前处理的组织切片形态质量令人满意后，随后将待处理的组织数量增加到B15、C20、D25、E30和F35。取4μm厚的切片，用苏木精和伊红染色法染色。对采用微波法和传统法处理的组织切片进行随机编号，用于盲法研究，由两名观察者独立评估。基于4个参数评估切片质量：细胞质细节、细胞核细节、组织结构和染色特征。使用SPSS 15.0进行统计分析。
当微波炉中的样本量（数量）达到25个样本时，家用微波处理（DMWTP）的形态学结果（质量）与传统处理（CTP）相当。
家用微波处理可有效地用于每次最大样本量为25个样本的实验室。其优点是速度快，形态质量与传统处理相同。
