Dorko E, Kmetová M, Dorko F, Bracoková I, Danko J, Svický E, Tkáciková L
Department of Physiology, Faculty of Medicine, Safárik University, 040 01 Kosice, Slovakia.
Folia Microbiol (Praha). 2000;45(4):369-72. doi: 10.1007/BF02817564.
Cryptococcus neoformans isolated from various clinical materials in 14 cases, was identified by (1) cultivation on Sabouraud glucose agar and CHROMagar Candida, (2) microscopic examination of Indian-ink-stained preparations and (3) determination of biochemical properties (assimilation and fermentation of saccharides, assimilation of KNO3, production of urease and phenol monooxygenase). C. neoformans was determined in five specimens from paediatric patients in the intensive care unit and in nine specimens from adult patients, most frequently from liquor at meningitis (n = 3).
从14例患者的各种临床样本中分离出新型隐球菌，通过以下方法进行鉴定：（1）在沙氏葡萄糖琼脂和科玛嘉念珠菌显色培养基上培养；（2）对印度墨汁染色标本进行显微镜检查；（3）测定生化特性（糖类的同化和发酵、硝酸钾的同化、脲酶和酚单加氧酶的产生）。在重症监护病房的5例儿科患者样本和9例成人患者样本中检测到新型隐球菌，最常见于脑膜炎患者的脑脊液样本（n = 3）。
