Lundström K H, Bamford D H, Palva E T, Lounatmaa K
J Gen Virol. 1979 Jun;43(3):583-92. doi: 10.1099/0022-1317-43-3-583.
The structure of the purified lipid-containing phage PR4 was studied electron microscopically using thin sectioning, negative staining and freeze-fracturing techniques. The lipid layer was located inside a rigid capsid and had a bilayer structure. The innermost dense core was probably composed solely of DNA since no major structural proteins were missing in empty phage particles lacking DNA. During infection, phages were attached to the cell wall of the host. They apparently inject their DNA through the cell envelope. The lipid layer of the phage might play an active role in the injection process. The maturation of the phage capsid takes place within the nuclear region of the cell, whereas intact phages with DNA were always seen in the cell periphery.
利用超薄切片、负染色和冷冻蚀刻技术，通过电子显微镜对纯化的含脂质噬菌体PR4的结构进行了研究。脂质层位于坚硬的衣壳内部，具有双层结构。最内层的致密核心可能仅由DNA组成，因为在缺乏DNA的空噬菌体颗粒中没有缺失主要结构蛋白。在感染过程中，噬菌体附着在宿主细胞壁上。它们显然通过细胞膜注入其DNA。噬菌体的脂质层可能在注入过程中起积极作用。噬菌体衣壳的成熟发生在细胞核区域内，而带有DNA的完整噬菌体总是出现在细胞周边。
