Cadden S P, Sands J A
Can J Microbiol. 1977 Aug;23(8):1084-7. doi: 10.1139/m77-163.
PR4 is a lipid-containing bacteriophage which is able to replicate in Escherichia coli. The virus was labeled with either [14C]leucine and [14C]threonine or H235SO4 and then purified by several rounds of sucrose gradient centrifugation. Autoradiographs showed the virus to be composed of six major protein species with molecular weights (1) 68 000, (2) 47 500, (3) 38 500, (4) 35 000, (5) 20 700, (6) 16 500 daltons. Electropherograms showed protein No. 2 to be the major protein, comprising about 43% of the total weight of viral protein.
PR4是一种含脂质的噬菌体，能够在大肠杆菌中复制。该病毒用[14C]亮氨酸、[14C]苏氨酸或H235SO4进行标记，然后通过几轮蔗糖梯度离心进行纯化。放射自显影片显示该病毒由六种主要蛋白质组成，分子量分别为：(1)68000、(2)47500、(3)38500、(4)35000、(5)20700、(6)16500道尔顿。电泳图谱显示2号蛋白质是主要蛋白质，约占病毒蛋白质总重量的43%。
