Pulvirenti T J, Yin J L, Chaufour X, McLachlan C, Hambly B D, Bennett M R, Barden J A
Institute for Biomedical Research and Department of Anatomy and Histology, The University of Sydney, N.S.W. 2006, Australia.
J Neurocytol. 2000 Sep;29(9):623-31. doi: 10.1023/a:1010828302936.
The redistribution of purinergic P2X receptor subunits (P2X(1) to P2X(7)) within the rabbit aorta wall three weeks after endothelial balloon injury/cholesterol feeding was examined. P2X(1) receptor cluster density was elevated in the media following balloon injury/cholesterol feeding by about 30% and these clusters appeared on smooth muscle cells throughout the greatly expanded neointima but they did not change significantly on the endothelial cells following balloon injury. P2X(4) clusters were found in high density throughout the media and in very high density in the enlarged neointima following balloon injury, particularly on the endothelial cells where the density increased about 10-fold after balloon injury. P2X(5) clusters were found in high density in the media of normal aorta but with little change following balloon injury. P2X(3), P2X(6) and P2X(7) cluster density was low in normal aorta and remained unchanged following balloon injury. All receptor subunits were found on endothelial cells. It is suggested that the release of ATP from damaged endothelial cells and from smooth muscle cells sufficient to activate P2X(4) receptors may contribute to neointimal proliferation.
研究了兔主动脉壁在球囊内皮损伤/胆固醇喂养三周后嘌呤能P2X受体亚基（P2X(1)至P2X(7)）的重新分布情况。球囊损伤/胆固醇喂养后，中膜内P2X(1)受体簇密度升高约30%，这些簇出现在整个大幅扩张的新生内膜的平滑肌细胞上，但球囊损伤后内皮细胞上的P2X(1)受体簇密度无显著变化。球囊损伤后，P2X(4)簇在整个中膜高密度分布，在扩大的新生内膜中密度极高，尤其是在内皮细胞上，球囊损伤后其密度增加约10倍。正常主动脉中膜P2X(5)簇密度高，但球囊损伤后变化不大。正常主动脉中P2X(3)、P2X(6)和P2X(7)簇密度低，球囊损伤后保持不变。在内皮细胞上均发现了所有受体亚基。提示受损内皮细胞和平滑肌细胞释放的三磷酸腺苷足以激活P2X(4)受体，这可能促进新生内膜增殖。
