Ashton F E, Caugant D A
Canadian Science Center for Human and Animal Health, Population and Public Health Branch, Winnipeg, MB.
Can J Microbiol. 2001 Apr;47(4):283-9.
Three hundred and one (301) strains of Neisseria meningitidis serogroup B, isolated from patients with meningococcal disease during the years 1994-1996, were subjected to multilocus enzyme electrophoresis, serotyping, and serosubtyping. Based on the analyses of 14 enzyme loci, 177 electrophoretic types (ETs) were identified. Of these, 136 were represented by single isolates and 41 were represented by multiple isolates (range 2-31). The mean genetic diversity for isolates was 0.444 and for ETs was 0.440. The index of association (I(A)) between loci was 0.530 +/- 0.08 for isolates and 0.256 +/- 0.10 for ETs. Cluster analysis revealed the presence of 39 lineages each represented by a single ET or clusters of ETs. The most common serotypes were 4, 15, and 14 and accounted for 84 (28.0%), 53 (17.6%), and 32 (10.6%) of the isolates, respectively, and were dispersed amongst 46 ETs (1-122), 35 ETs (3-165), and 26 ETs (18-76), respectively. The 109 (36.6%) nontypable (NT) isolates were amongst 74 ETs (6-177). The mean genetic diversity for serotypes 4, 15, and 14 and NT isolates was 0.368, 0.371, 0.343, and 0.442, respectively, and for ETs was 0.363, 0.354, 0.397, and 0.440, respectively. Combinations of serotypes and serosubtypes (number of isolates) that occurred most frequently were 4:P1.14 (17), 14:P1.16 (16), NT:P1.16 (16), 15:P1.16 (13), and NT:P1.13 (13). The majority of group B disease in Canada during 1994-1996 was caused by meningococci of considerable genetic diversity, and reflects a situation of endemic disease. However, the results also indicate that organisms belonging to the ET-5 complex, which has been responsible for outbreaks of group B disease globally for several decades, have been introduced into the country.
对1994年至1996年期间从脑膜炎球菌病患者中分离出的301株B群脑膜炎奈瑟菌进行多位点酶电泳、血清分型和血清亚型分析。基于对14个酶基因座的分析，鉴定出177种电泳类型（ETs）。其中，136种由单个分离株代表，41种由多个分离株代表（范围为2至31个）。分离株的平均遗传多样性为0.444，ETs的平均遗传多样性为0.440。分离株基因座之间的关联指数（I(A)）为0.530±0.08，ETs的为0.256±0.10。聚类分析显示存在39个谱系，每个谱系由单个ET或ETs簇代表。最常见的血清型为4、15和14，分别占分离株的84株（28.0%）、53株（17.6%）和32株（10.6%），分别分散在46个ETs（1至122）、35个ETs（3至165）和26个ETs（18至76）中。109株（36.6%）不可分型（NT）分离株分布在74个ETs（6至177）中。血清型4、15和14以及NT分离株的平均遗传多样性分别为0.368、0.371、0.343和0.442，ETs的分别为0.363、0.354、0.397和0.440。最常出现的血清型和血清亚型组合（分离株数量）为4:P1.14（17）、14:P1.16（16）、NT:P1.16（16）、15:P1.16（13）和NT:P1.13（13）。1994年至1996年期间加拿大的大多数B群疾病是由具有相当遗传多样性的脑膜炎球菌引起的，反映了地方病的情况。然而，结果还表明，几十年来在全球范围内导致B群疾病暴发的ET-5复合体的菌株已传入该国。
