Simar David, Malatesta Davide, Koechlin Christelle, Cristol Jean Paul, Vendrell Jean Pierre, Caillaud Corinne
EA 2991, Faculté des Sciences du Sport, 700 av du Pic Saint Loup, 34090 Montpellier, France.
Exp Gerontol. 2004 Oct;39(10):1467-74. doi: 10.1016/j.exger.2004.08.002.
Aging mammalians show reduced expression and induction of cytoprotective heat shock proteins in response to physiological stresses. Physical training can increase Hsp72 expression in young and old animals, but whether same adaptations can be observed in old people remains unknown. We hypothesized that the maintenance of physical activity during aging should preserve Hsp72 expression in leukocytes of elderly people. Intracellular and surface Hsp72 (the inducible form of Hsp70) expression in leukocytes as well as apoptotic and necrotic leukocytes were measured by flow cytometry at rest and after maximal incremental test on treadmill in the following groups: 8 young subjects (25.3+/-0.6 year, G25), 12 sexagenarians (66.2+/-1 year, G65) and 9 octogenarians (82.2+/-1.2 year, G85), all physically active. Protein and lipid oxidation markers were also measured at rest and post-exercise. Results showed significant lower basal percentages of Hsp72-positive lymphocytes in G85 compared to G25. At rest, lower mean fluorescence intensity in Hsp72-positive monocytes was measured in G65 and G85 compared to G25, and in granulocytes from G85 compared to G25. Maximal exercise test induced systemic oxidative-stress in the three groups but did not induce any increase in apoptotic or necrotic cells. We observed a significant increase in the percentage of Hsp72-positive lymphocytes from G85. This study showed that maintaining physical activity during aging can preserve the ability to induce Hsp72 in response to physiological stress.
衰老的哺乳动物在应对生理应激时，细胞保护性热休克蛋白的表达和诱导能力会降低。体育锻炼可以增加幼年和老年动物体内Hsp72的表达，但老年人是否也能观察到同样的适应性变化尚不清楚。我们推测，在衰老过程中保持身体活动应该能维持老年人白细胞中Hsp72的表达。通过流式细胞术测量了以下几组在静息状态下以及在跑步机上进行最大递增测试后白细胞内和表面Hsp72（Hsp70的可诱导形式）的表达，以及凋亡和坏死白细胞的情况：8名年轻受试者（25.3±0.6岁，G25组）、12名六旬老人（66.2±1岁，G65组）和9名八旬老人（82.2±1.2岁，G85组），所有受试者均有体育锻炼习惯。还在静息状态和运动后测量了蛋白质和脂质氧化标志物。结果显示，与G25组相比，G85组中Hsp72阳性淋巴细胞的基础百分比显著降低。静息状态下，与G25组相比，G65组和G85组中Hsp72阳性单核细胞的平均荧光强度较低，与G25组相比，G85组粒细胞中的平均荧光强度也较低。最大运动测试在三组中均诱发了全身氧化应激，但未导致凋亡或坏死细胞增加。我们观察到G85组中Hsp72阳性淋巴细胞的百分比显著增加。这项研究表明，在衰老过程中保持身体活动可以维持机体在生理应激下诱导Hsp72的能力。
