Christian M M, Moy R L, Wagner R F, Yen-Moore A
University of Texas Southwestern Medical Center, Dallas, Texas, University of California, USA.
Dermatol Surg. 2001 May;27(5):441-5. doi: 10.1046/j.1524-4725.2001.00200.x.
Actin is largely responsible for cell motility and is only sparsely found in normal epithelial cells. An altered expression of actin in some malignancies may facilitate aggressive invasion. Micronodular basal cell carcinoma (BCC) has been shown to require more surgical stages, wider tissue margins, and deeper defects for extirpation during Mohs micrographic surgery relative to nodular BCC.
To provide preliminary data regarding a possible correlation between alpha-smooth muscle actin (alpha-SMA) expression within the cells or stroma of micronodular BCC and aggressive invasion. In addition, the incidence of alpha-SMA expression in micronodular, morpheaform, and nodular BCC is evaluated.
Nine micronodular basal cell carcinomas (7 primary, 2 recurrent) were evaluated for neural invasion, depth of tissue invasion, and alpha smooth muscle actin antibodies. The presence of alpha-smooth muscle actin antibodies was assessed using immunoperoxidase staining and compared with 13 morpheaform (13 primary, 0 recurrent) and 12 nodular (12 primary, 0 recurrent).
Six of the nine micronodular (67%), eight of the 13 morpheaform (62%), and 0 of the 12 nodular (0%) BCCs stained positive for alpha-SMA. Of the six micronodular BCCs that stained positive for alpha-SMA, three invaded the fascia or muscle and three displayed neural invasion. In contrast, of the three micronodular BCCs that stained negative for alpha-SMA, none invaded the fascia or muscle and only one exhibited neural invasion.
Actin was present in 66% of micronodular, 62% of morpheaform, and 0% of nodular BCC. The presence of actin in micronodular BCC may be a marker for aggressive invasion.
肌动蛋白在很大程度上负责细胞运动，在正常上皮细胞中含量稀少。肌动蛋白在某些恶性肿瘤中的表达改变可能会促进侵袭性生长。与结节性基底细胞癌（BCC）相比，微结节性基底细胞癌在莫氏显微外科手术切除时需要更多的手术阶段、更宽的组织切缘和更深的缺损。
提供关于微结节性BCC细胞或基质中α-平滑肌肌动蛋白（α-SMA）表达与侵袭性生长之间可能相关性的初步数据。此外，评估微结节性、硬斑病样和结节性BCC中α-SMA表达的发生率。
对9例微结节性基底细胞癌（7例原发性，2例复发性）进行神经侵袭、组织侵袭深度和α-平滑肌肌动蛋白抗体评估。使用免疫过氧化物酶染色评估α-平滑肌肌动蛋白抗体的存在情况，并与13例硬斑病样（13例原发性，0例复发性）和12例结节性（12例原发性，0例复发性）进行比较。
9例微结节性BCC中有6例（67%）、13例硬斑病样BCC中有8例（62%）、12例结节性BCC中有0例（0%）α-SMA染色呈阳性。在6例α-SMA染色呈阳性的微结节性BCC中，3例侵犯筋膜或肌肉，3例显示神经侵袭。相比之下，在3例α-SMA染色呈阴性的微结节性BCC中，无一例侵犯筋膜或肌肉，仅1例表现出神经侵袭。
肌动蛋白存在于66%的微结节性、62%的硬斑病样和0%的结节性BCC中。微结节性BCC中肌动蛋白的存在可能是侵袭性生长的标志物。
