Ling C, Gao H, Chen Z
Department of Traditional Chinese Medicine, Changhai Hospital, Shanghai(200433).
Zhongguo Zhong Xi Yi Jie He Za Zhi. 1998 Jan;18(1):38-40.
To explore the mechanism of alleviating the side effects of irradiation by Sisheng Decoction (SSD).
The mice splenic lymphocytes were treated by hydroxylurea to stop the DNA replication in S stage, irradiated by ultraviolet ray (UV) and the 3H TdR was added into the cultural medium. After the lymphocytes were cultured in 37 degrees C for 4 h, the capacity for 3H-TdR in the lymphocytes were tested. The effect of SSD in different content on the cut and patch repair(CPR) of DNA can be inferred by observing the lymphocyte uptake index (LUI) of 3H-TdR.
LUI of mice spleen lymphocytes could be increased by pretreating the lymphocytes with SSD for 4 h and then irradiated by UV, but it could not be increased if the lymphocytes were treated by SSD and UV at the same time.
SSD can alleviate the side effect of irradiation by enhancing the function of CPR-DNA, which need pretreating the lymphocytes with SSD.
探讨四生汤减轻辐射副作用的机制。
用羟基脲处理小鼠脾淋巴细胞以阻断S期DNA复制，经紫外线（UV）照射，同时在培养基中加入3H-TdR。淋巴细胞在37℃培养4小时后，检测淋巴细胞中3H-TdR的摄取能力。通过观察3H-TdR的淋巴细胞摄取指数（LUI），可推断不同含量的四生汤对DNA切割及修复（CPR）的影响。
先用四生汤预处理小鼠脾淋巴细胞4小时后再进行UV照射，可使LUI升高；而若淋巴细胞同时用四生汤和UV处理，则LUI无升高。
四生汤可通过增强DNA的CPR功能减轻辐射副作用，且需要先用四生汤预处理淋巴细胞。
