Augé-Gouillou C, Hamelin M H, Demattei M V, Periquet M, Bigot Y
IRBI-Groupe d'Etude des Parasites Génétiques, UPRESA CNRS 6035, Faculté des Sciences, Tours, France.
Mol Genet Genomics. 2001 Mar;265(1):51-7. doi: 10.1007/s004380000385.
The two inverted terminal repeats (ITRs) flanking the Mos-1 mariner element differ in sequence at four positions. Gel retardation experiments indicated that each of these differences has a significant impact on the quality of the interaction between the ITR and the Mos-1 transposase. We showed that the transposase binds to the 3' ITR better than to the 5' ITR. The results of transposition assays performed in Escherichia coli indicated that these differences have an influence on the rate of transposition and the stability of the transposition products. Finally, we find that the wild-type configuration of the Mos-1 element, with one 5' ITR and one 3' ITR, is less efficient for transposition in bacteria than that of an element having two 3' ITRs.
位于Mos-1水手元件两侧的两个反向末端重复序列（ITRs）在四个位置上存在序列差异。凝胶阻滞实验表明，这些差异中的每一个都对ITR与Mos-1转座酶之间相互作用的质量有显著影响。我们发现转座酶与3' ITR的结合比与5' ITR的结合更好。在大肠杆菌中进行的转座分析结果表明，这些差异对转座速率和转座产物的稳定性有影响。最后，我们发现具有一个5' ITR和一个3' ITR的Mos-1元件的野生型构型在细菌中的转座效率低于具有两个3' ITR的元件。
