Rodrigues F, van Hemert M, Steensma H Y, Côrte-Real M, Leão C
Centro de Ciências do Ambiente, Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
J Bacteriol. 2001 Jun;183(12):3791-4. doi: 10.1128/JB.183.12.3791-3794.2001.
We describe the utilization of a red fluorescent protein (DsRed) as an in vivo marker for Saccharomyces cerevisiae. Clones expressing red and/or green fluorescent proteins with both cytoplasmic and nuclear localization were obtained. A series of vectors are now available which can be used to create amino-terminal (N-terminal) and carboxyl-terminal (C-terminal) fusions with the DsRed protein.
我们描述了利用红色荧光蛋白(DsRed)作为酿酒酵母的体内标记。获得了表达具有细胞质和细胞核定位的红色和/或绿色荧光蛋白的克隆。现在有一系列载体可用于创建与DsRed蛋白的氨基末端(N末端)和羧基末端(C末端)融合体。