在活的出芽酵母细胞中成像肌动蛋白细胞骨架。
Imaging the Actin Cytoskeleton in Live Budding Yeast Cells.
机构信息
Department of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY, USA.
Confocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY, USA.
出版信息
Methods Mol Biol. 2022;2364:53-80. doi: 10.1007/978-1-0716-1661-1_3.
Although budding yeast, Saccharomyces cerevisiae, is widely used as a model organism in biological research, studying cell biology in yeast was hindered due to its small size, rounded morphology, and cell wall. However, with improved techniques, researchers can acquire high-resolution images and carry out rapid multidimensional analysis of a yeast cell. As a result, imaging in yeast has emerged as an important tool to study cytoskeletal organization, function, and dynamics. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in live yeast cells.
虽然 budding yeast（出芽酵母），Saccharomyces cerevisiae（酿酒酵母），被广泛用作生物研究中的模式生物，但由于其体积小、形态圆润和细胞壁的存在，研究酵母细胞生物学受到了阻碍。然而，随着技术的改进，研究人员可以获得酵母细胞的高分辨率图像并进行快速多维分析。因此，酵母细胞成像已成为研究细胞骨架组织、功能和动力学的重要工具。本章介绍了在活酵母细胞中可视化肌动蛋白细胞骨架的技术和方法。
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