Frank W E, Llewellyn B E, Fish P A, Riech A K, Marcacci T L, Gandor D W, Parker D, Carter R R, Thibault S M
Illinois State Police, Research and Development Laboratory, Springfield 62702, USA.
J Forensic Sci. 2001 May;46(3):642-6.
According to TWGDAM guideline 4.5 (1), prior to implementing a new DNA analysis procedure or an existing DNA analysis procedure developed by another laboratory, the forensic laboratory must first demonstrate reliability of the procedure in-house. Seven phases were designed to validate the use of the AmpFlSTR Profiler Plus PCR Amplification Kit, as well as the PE Applied Biosystems 310 Genetic Analyzer. This report summarizes the results obtained for each of the seven phases of the validation study which included the following evaluations: polymer, reproducibility, sensitivity, stutter, precision, mixtures and nonprobative casework.
根据TWGDAM指南4.5(1)，在实施新的DNA分析程序或另一个实验室开发的现有DNA分析程序之前，法医实验室必须首先在内部证明该程序的可靠性。设计了七个阶段来验证AmpFlSTR Profiler Plus PCR扩增试剂盒以及PE Applied Biosystems 310基因分析仪的使用。本报告总结了验证研究七个阶段中每个阶段获得的结果，其中包括以下评估：聚合、再现性、灵敏度、拖尾、精密度、混合样本和无证明力的案例分析。
