用于测定厌氧菌抗生素敏感性的肉汤稀释法。
Broth-dilution method for determining the antibiotic susceptibility of anaerobic bacteria.
作者信息
Stalons D R, Thornsberry C
出版信息
Antimicrob Agents Chemother. 1975 Jan;7(1):15-21. doi: 10.1128/AAC.7.1.15.
Abstract
A broth-dilution method for performing antimicrobial susceptibility tests on anaerobic bacteria has been proposed. The medium used in the test was Schaedler broth, with incubation in a glove box with an atmosphere of 5% CO(2), 10% H(2), and 85% N(2), or in the GasPak system. Minimal inhibitory concentrations for selected antibiotics were determined, under these conditions, by using a conventional twofold dilution scheme for the antibiotics and a "categorization three-tube method" in which two or three clinically significant concentrations of each antibiotic were used. Minimal inhibitory concentrations obtained by both methods were very similar. The categorization method could be used routinely to test the antimicrobial susceptibility of anaerobic bacteria.
摘要
已提出一种用于对厌氧菌进行抗菌药敏试验的肉汤稀释法。试验中使用的培养基是 Schaedler 肉汤，在含 5%二氧化碳、10%氢气和 85%氮气的手套箱中培养，或在 GasPak 系统中培养。在这些条件下，通过使用常规的抗生素两倍稀释方案和“分类三管法”（即每种抗生素使用两到三个具有临床意义的浓度）来确定所选抗生素的最低抑菌浓度。两种方法获得的最低抑菌浓度非常相似。分类法可常规用于检测厌氧菌的抗菌药敏性。
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