Kiehn T E, Capitolo C, Armstrong D
J Clin Microbiol. 1982 Jul;16(1):96-8. doi: 10.1128/jcm.16.1.96-98.1982.
Turbid broth (0.5 ml) from blood culture bottles was inoculated into 0.5 ml of brain heart infusion broth, incubated for 3 to 6 h, diluted 1:500 in distilled water, and then inoculated directly into microtiter broth dilution susceptibility trays to test for minimal inhibitory concentrations. The results were compared to the standard tests performed 24 h later on colonies from subculture plates. The minimal inhibitory concentrations measured by these two methods were compared in 1,875 organism-antibiotic tests. The two minimal inhibitory concentrations were identical in 86.0% and within one twofold dilution in 98.0% of the tests. An organism was judged to be susceptible by one method and resistant by the other in 13 tests (0.7%). These 13 discrepancies were distributed among several organism-antibiotic combinations; no more than two were seen for any one combination. Highly accurate susceptibility testing can be achieved by using direct inoculation of turbid blood culture broths.
将血培养瓶中的浑浊肉汤（0.5 ml）接种到0.5 ml脑心浸液肉汤中，培养3至6小时，用蒸馏水按1:500稀释，然后直接接种到微量肉汤稀释药敏试验板中以检测最低抑菌浓度。将结果与24小时后对传代培养平板上的菌落进行的标准试验结果进行比较。在1875次微生物-抗生素试验中比较了这两种方法测得的最低抑菌浓度。两种最低抑菌浓度在86.0%的试验中相同，在98.0%的试验中相差不超过一倍稀释度。在13次试验（0.7%）中，一种方法判断为敏感而另一种方法判断为耐药。这13个差异分布在几种微生物-抗生素组合中；任何一种组合中出现的差异不超过两个。通过直接接种浑浊的血培养肉汤可以实现高度准确的药敏试验。
