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厌氧菌抗生素敏感性测试的标准化单碟法

Standardized single-disc method for antibiotic susceptibility testing of anaerobic bacteria.

作者信息

Wikins T D, Holdeman L V, Abramson I J, Moore W E

出版信息

Antimicrob Agents Chemother. 1972 Jun;1(6):451-9. doi: 10.1128/AAC.1.6.451.

Abstract

A method was developed for determination of the antibiotic susceptibility of anaerobic bacteria by use of a single-disc diffusion technique and incorporation of the inoculum in pour plates. The method was standardized by correlation of zone diameters with minimal inhibitory concentrations determined in broth. Zone diameters could be used to approximate the minimal inhibitory concentrations of the seven antibiotics tested: ampicillin, bacitracin, carbenicillin, cephalothin, clindamycin, penicillin, and tetracycline.

摘要

开发了一种通过单碟扩散技术并将接种物掺入倾注平板来测定厌氧菌抗生素敏感性的方法。通过将抑菌圈直径与肉汤中测定的最低抑菌浓度进行相关性分析,对该方法进行了标准化。抑菌圈直径可用于估算所测试的七种抗生素的最低抑菌浓度:氨苄青霉素、杆菌肽、羧苄青霉素、头孢噻吩、克林霉素、青霉素和四环素。

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