Ebert B, Sukowski U, Grosenick D, Wabnitz H, Moesta K T, Licha K, Becker A, Semmler W, Schlag P M, Rinneberg H
Physikalisch-Technische Bundesanstalt, Abbestrasse 2-12, D-10587 Berlin, Germany.
J Biomed Opt. 2001 Apr;6(2):134-40. doi: 10.1117/1.1350561.
Optical mammography with near-infrared (NIR) light using time-domain, frequency-domain, or continuous-wave techniques is a novel imaging modality to locate human breast tumors. By investigating excised specimens of normal and diseased mamma tissue we were able to demonstrate that differences in their scattering properties are a poor predictive parameter for normal and diseased mamma tissue. This paper describes the application of a NIR dye to improve the differentiation between breast tumors and normal tissue in a rat model. The NIR dye furnished a high tumor-to-tissue contrast ratio (6:1) in fluorescence images. Furthermore, this dye was used to develop liquid scattering phantoms with absorbing and fluorescent inhomogeneities. Using frequency-domain and time-domain instrumentation these inhomogeneities were localized at sufficient contrast by their increased absorption and fluorescence. Contrast between inhomogeneities and surrounding medium could be improved by combining fluorescence and transmittance images.
采用时域、频域或连续波技术的近红外光光学乳腺成像，是一种用于定位人类乳腺肿瘤的新型成像方式。通过研究正常和病变乳腺组织的切除标本，我们能够证明，它们散射特性的差异对于正常和病变乳腺组织而言，并不是一个很好的预测参数。本文描述了一种近红外染料在大鼠模型中用于改善乳腺肿瘤与正常组织之间区分度的应用。这种近红外染料在荧光图像中提供了高肿瘤与组织对比度（6:1）。此外，该染料被用于开发具有吸收和荧光不均匀性的液体散射体模。利用频域和时域仪器，这些不均匀性因其吸收和荧光增加而以足够的对比度被定位。通过结合荧光和透射率图像，可以提高不均匀性与周围介质之间的对比度。
