Mustari Afrina, Nishidate Izumi, Wares Md Abdul, Maeda Takaaki, Kawauchi Satoko, Sato Shunichi, Sato Manabu, Aizu Yoshihisa
Graduate School of Bio-application & Systems Engineering, Tokyo University of Agriculture & Technology.
Graduate School of Bio-application & Systems Engineering, Tokyo University of Agriculture & Technology;
J Vis Exp. 2018 Aug 22(138):57578. doi: 10.3791/57578.
This protocol describes how to make agarose-based tissue-mimicking phantoms and demonstrates how to determine their optical properties using a conventional optical system with an integrating sphere. Measuring systems for the acquisition of the diffuse reflectance and total transmittance spectra are constructed with a broadband white light source, a light guide, an achromatic lens, an integrating sphere, a sample holder, an optical fiber probe, and a multi-channel spectrometer. An acrylic mold consisting of two rectangular acrylic pieces and a U-shaped acrylic piece is constructed to create an epidermal phantom and a dermal phantom with whole blood. The application of a sodium dithionite (Na2S2O4) solution to the dermal phantom enables the researcher to deoxygenate hemoglobin in red blood cells distributed in the dermal phantom. The inverse Monte Carlo simulation with the diffuse reflectance and total transmittance spectra measured by a spectrometer with an integrating sphere is performed to determine the absorption coefficient spectrum µa(λ) and the reduced scattering coefficient spectrum µs'(λ) of each layer phantom. A two-layered phantom mimicking the diffuse reflectance of human skin tissue is also demonstrated by piling up the epidermal phantom on the dermal phantom.
本方案描述了如何制作基于琼脂糖的组织模拟体模，并展示了如何使用带有积分球的传统光学系统来测定其光学特性。用于采集漫反射和总透射光谱的测量系统由宽带白光源、光导、消色差透镜、积分球、样品架、光纤探头和多通道光谱仪构成。制作了一个由两块矩形丙烯酸片和一块U形丙烯酸片组成的丙烯酸模具，用于创建带有全血的表皮体模和真皮体模。向真皮体模中加入连二亚硫酸钠（Na2S2O4）溶液，可使研究人员对分布在真皮体模中的红细胞内的血红蛋白进行脱氧。利用带有积分球的光谱仪测量的漫反射和总透射光谱进行逆蒙特卡罗模拟，以确定各层体模的吸收系数光谱µa(λ)和约化散射系数光谱µs'(λ)。通过将表皮体模堆叠在真皮体模上，还展示了一种模拟人体皮肤组织漫反射的双层体模。
