通过采用改变的标本运送方法和高质量的宫颈管内标本，改进沙眼衣原体的聚合酶链反应检测。
Improved PCR detection of Chlamydia trachomatis by using an altered method of specimen transport and high-quality endocervical specimens.
作者信息
Kellogg J A, Seiple J W, Klinedinst J L, Stroll E S, Cavanaugh S H
机构信息
Department of Pathology, York Hospital, Pennsylvania 17405, USA.
出版信息
J Clin Microbiol. 1995 Oct;33(10):2765-7. doi: 10.1128/jcm.33.10.2765-2767.1995.
Abstract
Duplicate endocervical swabs were collected for detection of Chlamydia trachomatis by PCR (Roche Diagnostics). One swab was swirled in Specimen Transport Medium (Roche) for PCR testing and discarded. A saline aliquot from the other specimen, sent as a dry swab to the laboratory, was Papanicolaou stained to determine specimen adequacy, and the remainder was PCR tested. Significantly more (24%) PCR-positive results (118 versus 95; P < 0.001) were obtained with the dry specimens than with the swirled specimens when first tested. In addition, PCR-positive results were obtained with 107 (10.6%) of 1,007 microscopically adequate specimens but with only 3 (0.9%) of 341 inadequate specimens (P < 0.001).
摘要
采集两份宫颈拭子，通过聚合酶链反应（PCR，罗氏诊断公司）检测沙眼衣原体。一份拭子在标本运输培养基（罗氏）中涡旋以进行PCR检测，然后丢弃。另一份标本作为干燥拭子送至实验室，取其一份盐水样本进行巴氏染色以确定标本是否合格，其余部分进行PCR检测。首次检测时，干燥标本获得的PCR阳性结果（118例对95例；P<0.001）明显多于涡旋标本。此外，在1007例显微镜检查合格的标本中，有107例（10.6%）获得PCR阳性结果，但在341例不合格标本中，只有3例（0.9%）获得阳性结果（P<0.001）。
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