Ching S, Lee H, Hook E W, Jacobs M R, Zenilman J
Diagnostics Division, Abbott Laboratories, Abbott Park, Illinois 60064, USA.
J Clin Microbiol. 1995 Dec;33(12):3111-4. doi: 10.1128/jcm.33.12.3111-3114.1995.
The ligase chain reaction (LCR) is an in vitro nucleic acid amplification technique that exponentially amplifies targeted DNA sequences. In a multicenter study, we evaluated the use of a 4-h LCR-based assay for the diagnosis of Neisseria gonorrhoeae infection of the cervix and male urethra. The LCR results were compared with those of culture for N. gonorrhoeae by using selective media. This assay amplifies target sequences within the N. gonorrhoeae opacity gene. Discordant LCR-positive and culture-negative specimens were further evaluated by testing by another LCR assay which used N. gonorrhoeae-specific pilin probe sets. A total of 1,539 female endocervical specimens and 808 male urethral swab specimens were evaluated in the study. An expanded "gold standard" was defined to include all culture-positive as well as culture-negative, confirmed LCR-positive specimens. After resolution of discrepant samples, the sensitivities of the N. gonorrhoeae LCR assays for the female and male specimens were 97.3 and 98.5%, respectively, with specificities of 99.6 and 99.8%, respectively. Resolved culture sensitivities were 83.9 and 96.5% for the female and male specimens, respectively. The LCR assay for gonorrhea is a rapid, highly sensitive nonculture method for detecting gonococcal infection of the cervix and male urethra.
连接酶链式反应（LCR）是一种体外核酸扩增技术，可对数扩增靶向DNA序列。在一项多中心研究中，我们评估了基于LCR的4小时检测法用于诊断宫颈和男性尿道淋病奈瑟菌感染的情况。通过使用选择性培养基，将LCR结果与淋病奈瑟菌培养结果进行比较。该检测法扩增淋病奈瑟菌不透明蛋白基因内的靶序列。对LCR阳性而培养阴性的不一致标本，通过使用淋病奈瑟菌特异性菌毛蛋白探针组的另一种LCR检测法进行进一步评估。本研究共评估了1539份女性宫颈标本和808份男性尿道拭子标本。定义了一个扩展的“金标准”，包括所有培养阳性以及培养阴性但经确认LCR阳性的标本。在解决了有差异的样本后，淋病奈瑟菌LCR检测法对女性和男性标本的敏感性分别为97.3%和98.5%，特异性分别为99.6%和99.8%。解决后培养法对女性和男性标本的敏感性分别为83.9%和96.5%。用于淋病的LCR检测法是一种快速、高度灵敏的非培养方法，用于检测宫颈和男性尿道的淋球菌感染。
