Shafii A, Sopher S, Lev M, Das K M
Lancet. 1981 Aug 15;2(8242):332-4. doi: 10.1016/s0140-6736(81)90648-6.
Sera from patients with Crohn's disease or ulcerative colitis, and from controls were examined by indirect immunofluorescence for antibody against two strains of pseudomonas-like cell-wall-defective bacterial variants. Serum samples from 22 of 25 patients with Crohn's disease produced fluorescence of both revertant cell-wall-defective bacterial strains. Intensity of fluorescence correlated positively with the degree of disease activity. Sera from 23 patients with ulcerative colitis and from 15 control subjects did not produce any significant staining of either of the two revertant cell-wall-defective bacterial strains. Absorption of sera with Escherichia coli, Bacteroides thetaiotaomicron, and Pseudomonas aeruginosa did not alter the intensity of fluorescence in patients with Crohn's disease, whereas similar absorption of sera from patients with ulcerative colitis and controls abolished the slight staining of cell-wall-defective strains produced by 29% of unabsorbed serum samples.
采用间接免疫荧光法检测克罗恩病或溃疡性结肠炎患者以及对照者血清中针对两株假单胞菌样细胞壁缺陷细菌变体的抗体。25例克罗恩病患者中的22例血清样本对两种回复型细胞壁缺陷细菌菌株均产生荧光。荧光强度与疾病活动程度呈正相关。23例溃疡性结肠炎患者和15例对照者的血清对两种回复型细胞壁缺陷细菌菌株均未产生任何明显染色。用大肠杆菌、嗜水气单胞菌和铜绿假单胞菌吸收血清后，克罗恩病患者血清的荧光强度未改变，而溃疡性结肠炎患者和对照者血清经类似吸收后，消除了29%未吸收血清样本对细胞壁缺陷菌株产生的轻微染色。
