Sunna A, Gibbs M D, Bergquist P L
Department of Biological Sciences, Macquarie University, Sydney, N.S.W. 2109, Australia.
Biochem J. 2001 Jun 15;356(Pt 3):791-8. doi: 10.1042/0264-6021:3560791.
Many glycoside hydrolases, which degrade long-chain carbohydrate polymers, possess distinct catalytic modules and non-catalytic carbohydrate-binding modules (CBMs). On the basis of conserved protein secondary structure, we describe here the identification and experimental characterization of novel type of mannanase-associated mannan-binding module and also characterization of two CBM family 4 laminarinase-associated beta-glucan-binding modules. These modules are predicted to belong to a superfamily of CBMs which include families 4, 16, 17, 22 and a proposed new family, family 27.
许多可降解长链碳水化合物聚合物的糖苷水解酶都拥有独特的催化模块和非催化性碳水化合物结合模块(CBMs)。基于保守的蛋白质二级结构,我们在此描述了一种新型甘露聚糖酶相关甘露聚糖结合模块的鉴定及实验表征,以及两种CBM家族4的海带多糖酶相关β-葡聚糖结合模块的表征。这些模块预计属于一个CBM超家族,该超家族包括4、16、17、22家族以及一个新提出的家族——27家族。