van Gulik W M., ten Hoopen H J.G., Heijnen J J.
Biotechnology Delft-Leiden, Project Group Plant Cell Biotechnology, Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, The, Delft, Netherlands
Enzyme Microb Technol. 2001 Jun 7;28(9-10):796-805. doi: 10.1016/s0141-0229(01)00331-3.
Continuous culture of plant cell suspensions has been developed during the last 35 years. Starting from rather imperfect set-ups, nowadays much better equipment is used for studies on growth and production kinetics or cell physiology. In this review the development of equipment and theory, as well as the applications are discussed.
在过去35年里,植物细胞悬浮液的连续培养技术得到了发展。从相当不完善的装置起步,如今人们使用了更好的设备来研究生长、生产动力学或细胞生理学。在这篇综述中,我们将讨论设备和理论的发展以及其应用。