成纤维细胞生长因子-1和成纤维细胞生长因子-2调节胰腺腺癌细胞系中的E-钙黏蛋白/连环蛋白系统。
FGF-1 and FGF-2 modulate the E-cadherin/catenin system in pancreatic adenocarcinoma cell lines.
作者信息
El-Hariry I, Pignatelli M, Lemoine N R
机构信息
Imperial Cancer Research Fund Molecular Oncology Unit, Imperial College School of Medicine, London, UK.
出版信息
Br J Cancer. 2001 Jun 15;84(12):1656-63. doi: 10.1054/bjoc.2001.1813.
Abstract
Fibroblast growth factors (FGFs) and fibroblast growth factor receptors (FGFRs) have been increasingly recognized to play an important role in the pathobiology of pancreatic malignancy. We have investigated the effects of FGF-1 and FGF-2 on the behaviour and adhesion properties of human pancreatic adenocarcinoma cell lines (BxPc3, T3M4 and HPAF) that were previously characterised for the expression of FGFRs. Here we show that exposure to FGF-1 and FGF-2 leads to significant and dose-dependent increase in E-cadherin-dependent cell-cell adhesion, tubular differentiation, and a reduced capacity to invade collagen gels. FGF stimulation produces phosphorylation of E-cadherin and beta-catenin on tyrosine residues, as well as increased E-cadherin localisation to the cytoplasmic membrane and association with FGFR1 demonstrable by coimmunoprecipitation. These results demonstrate that FGF-1 and FGF-2 may be involved in the regulation of cell adhesion, differentiation and invasion of pancreatic cancer.
摘要
成纤维细胞生长因子（FGFs）和成纤维细胞生长因子受体（FGFRs）在胰腺恶性肿瘤的病理生物学中发挥重要作用，这一点已得到越来越多的认可。我们研究了FGF-1和FGF-2对人胰腺腺癌细胞系（BxPc3、T3M4和HPAF）行为和黏附特性的影响，这些细胞系之前已对FGFRs的表达进行了表征。在此我们表明，暴露于FGF-1和FGF-2会导致E-钙黏蛋白依赖性细胞间黏附、管状分化显著且呈剂量依赖性增加，以及侵袭胶原凝胶的能力降低。FGF刺激会使E-钙黏蛋白和β-连环蛋白的酪氨酸残基发生磷酸化，同时E-钙黏蛋白在细胞质膜上的定位增加，并通过免疫共沉淀证明其与FGFR1相关。这些结果表明，FGF-1和FGF-2可能参与了胰腺癌细胞黏附、分化和侵袭的调控。
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本文引用的文献
1
Upregulation of basic fibroblast growth factor in breast carcinoma and its relationship to vascular density, oestrogen receptor, epidermal growth factor receptor and survival.乳腺癌中碱性成纤维细胞生长因子的上调及其与血管密度、雌激素受体、表皮生长因子受体和生存的关系。
Ann Oncol. 1999 Jun;10(6):707-13. doi: 10.1023/a:1008303614441.
2
Characterization of the E-cadherin-catenin complexes in pancreatic carcinoma cell lines.胰腺癌细胞系中E-钙黏蛋白-连环蛋白复合物的特征分析
J Pathol. 1999 Jun;188(2):155-62. doi: 10.1002/(SICI)1096-9896(199906)188:2<155::AID-PATH356>3.0.CO;2-Y.
3
Changes in the expression of syndecan-1 in the colorectal adenoma-carcinoma sequence.
Virchows Arch. 1999 Feb;434(2):121-5. doi: 10.1007/s004280050315.
4
Basic fibroblast growth factor receptors and their prognostic value in human breast cancer.碱性成纤维细胞生长因子受体及其在人类乳腺癌中的预后价值。
Clin Cancer Res. 1998 Dec;4(12):2939-47.
5
Tyrosine phosphorylation and src family kinases control keratinocyte cell-cell adhesion.酪氨酸磷酸化和Src家族激酶控制角质形成细胞间的细胞黏附。
J Cell Biol. 1998 Jun 15;141(6):1449-65. doi: 10.1083/jcb.141.6.1449.
6
The epidermal growth factor receptor modulates the interaction of E-cadherin with the actin cytoskeleton.表皮生长因子受体调节E-钙黏蛋白与肌动蛋白细胞骨架的相互作用。
J Biol Chem. 1998 Apr 10;273(15):9078-84. doi: 10.1074/jbc.273.15.9078.
7
8
Cell scattering of SK-N-MC neuroepithelioma cells in response to Ret and FGF receptor tyrosine kinase activation is correlated with sustained ERK2 activation.SK-N-MC神经上皮瘤细胞响应Ret和FGF受体酪氨酸激酶激活的细胞散射与持续的ERK2激活相关。
Oncogene. 1997 Mar 13;14(10):1147-57. doi: 10.1038/sj.onc.1200911.
9
Expression of a dominant negative FGF receptor inhibits axonal growth and FGF receptor phosphorylation stimulated by CAMs.显性负性成纤维细胞生长因子（FGF）受体的表达抑制了细胞粘附分子（CAMs）刺激的轴突生长和FGF受体磷酸化。
Neuron. 1997 Feb;18(2):231-42. doi: 10.1016/s0896-6273(00)80264-0.
10
Integrins can collaborate with growth factors for phosphorylation of receptor tyrosine kinases and MAP kinase activation: roles of integrin aggregation and occupancy of receptors.整合素可与生长因子协同作用，使受体酪氨酸激酶磷酸化并激活丝裂原活化蛋白激酶：整合素聚集和受体占据的作用。
J Cell Biol. 1996 Dec;135(6 Pt 1):1633-42. doi: 10.1083/jcb.135.6.1633.
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