Lai X H, Golovliov I, Sjöstedt A
Department of Clinical Microbiology, Clinical Bacteriology, Umeå University, SE-901 85 Umeå, Sweden.
Infect Immun. 2001 Jul;69(7):4691-4. doi: 10.1128/IAI.69.7.4691-4694.2001.
The murine macrophage-like cell line J774.A1 ingests and allows intracellular growth of Francisella tularensis. We demonstrate that, after 24 h of infection, a pronounced cytopathogenicity resulted and the J774 cells were undergoing apoptosis. Despite this host cell apoptosis, no decrease in bacterial numbers was observed. When internalization of bacteria was prevented or intracellularly located F. tularensis bacteria were eradicated within 12 h, the progression of host cell cytopathogenicity and apoptosis was prevented.
鼠巨噬细胞样细胞系J774.A1摄取土拉弗朗西斯菌并允许其在细胞内生长。我们证明，感染24小时后，出现了明显的细胞致病性，J774细胞正在发生凋亡。尽管宿主细胞发生凋亡，但未观察到细菌数量减少。当细菌的内化被阻止或细胞内定位的土拉弗朗西斯菌在12小时内被根除时，宿主细胞细胞致病性和凋亡的进程被阻止。
