Ha T
Department of Physics and Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Curr Opin Struct Biol. 2001 Jun;11(3):287-92. doi: 10.1016/s0959-440x(00)00204-9.
Single-molecule fluorescence methods and biomechanical tools provide exciting new opportunities to probe biochemical processes in unprecedented detail. The detection and spectroscopy of single fluorophores have recently been used to observe conformational changes and biochemical events involving nucleic acids. A number of fluorescence observables, including localization, quenching, polarization response and fluorescence resonance energy transfer, have been utilized. An exciting new opportunity of combining fluorescence methods and biomechanical tools to study the structural changes and functions of enzymes that participate in nucleic acid metabolism has also arisen.
单分子荧光方法和生物力学工具为以前所未有的细节探究生化过程提供了令人兴奋的新机会。单荧光团的检测和光谱学最近已被用于观察涉及核酸的构象变化和生化事件。包括定位、猝灭、偏振响应和荧光共振能量转移在内的许多荧光可观测物已得到应用。将荧光方法和生物力学工具相结合来研究参与核酸代谢的酶的结构变化和功能这一令人兴奋的新机会也已出现。
